Barbara of Brandenburg (May 30, 1464 – September 4, 1515), is a German noblewoman member of the House of Hohenzollern, by birth Margravine of Brandenburg and by her two marriages Duchess of Głogów and Queen of Bohemia.
She was born in Ansbach as the tenth of the nineteen children of Albrecht III Achilles, Margrave and from 1471 Elector of Brandenburg; however she is the fourth child born from his second marriage to Anna of Saxony.